R. Amanda C. Larue

Title
InstitutionMedical University of South Carolina
DepartmentPathology and Laboratory Medicine
AddressP.O. Box MSC 908
432
114 Doughty St.
Phone843-789-6713
Fax843-876-5381
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    Collapse Biography 
    Collapse education and training
    Wofford College, Spartanburg, SCBA, BS05/1997Biology and French
    Medical University of South Carolina, Charleston, SCPhD05/2003Molecular, Cellular, and Pathobiology

    Collapse Overview 
    Collapse overview
    Research efforts in my laboratory are focused on the physio-pathological potential of hematopoietic stem cells (HSCs). The adult bone marrow is thought to contain at least two discernible stem cell populations: HSCs, defined by their capability of hematopoietic reconstitution in vivo, and mesenchymal stromal cells (MSC), originally defined by their in vitro potential to differentiate into mesenchymal tissue cells including fibroblasts, fat, cartilage, and bone. Recent studies suggest that organs once thought to be incapable of regenerative ability may indeed be capable of regeneration and repair via stem cells. While the potential of stem cells is clear from studies in which unfractionated or unpurified populations of bone marrow are transplanted, due to technical challenges, controversies have resulted when attempts are made to assign specific potentials to HSCs or MSCs. This has led to the need for stringent, well-defined lineage tracing methods. To this end, my laboratory has developed a transplantation model in which the bone marrow of lethally irradiated recipient mice is reconstituted by a clonal population of cells derived from a single HSC that expresses enhanced green fluorescent protein. This transplantation model is technically complex and experimentally challenging, with only a few laboratories worldwide capable of executing such a transplantation scheme. Our work also employs novel transgenic models to discern HSC-derived cells in tissues. With an eye towards translational potential, we also employ xenograft models to investigate the role(s) of human HSCs. The development of these tools has allowed my laboratory, my mentees, and my collaborators to conduct paradigm-shifting studies of the roles of HSCs in health and disease. Together, these approaches have resulted in discovery of novel roles for HSC and HSC-derived cells in a variety of pathological conditions that directly impact human health, including cancer, cardiovascular disease, pulmonary fibrosis, and orthopedic injury and repair.
    Collapse keywords
    Stem cell, Hematopoietic stem cell, mesenchymal stem cell, immunology, pathology, cancer, orthopedic, pulmonary, cardiovascular, PTSD

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    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. Adu-Ampratwum D, Pan Y, Koneru PC, Antwi J, Hoyte AC, Kessl J, Griffin PR, Kvaratskhelia M, Fuchs JR, Larue RC. Identification and Optimization of a Novel HIV-1 Integrase Inhibitor. ACS Omega. 2022 Feb 08; 7(5):4482-4491. PMID: 35155940.
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    2. Vilmen G, Smith AC, Benet HC, Shukla RK, Larue RC, Herschhorn A, Sharma A. Conformation of HIV-1 Envelope Governs Rhesus CD4 Usage and Simian-Human Immunodeficiency Virus Replication. mBio. 2022 Jan 11; e0275221. PMID: 35012342.
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    3. Rabe AJ, Tan YY, Larue RC, Yoder KE. Prototype Foamy Virus Integrase Displays Unique Biochemical Activities among Retroviral Integrases. Biomolecules. 2021 12 20; 11(12). PMID: 34944553.
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    4. Weissman JD, Singh AK, Devaiah BN, Schuck P, LaRue RC, Singer DS. The intrinsic kinase activity of BRD4 spans its BD2-B-BID domains. J Biol Chem. 2021 11; 297(5):101326. PMID: 34688663.
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    5. Xing E, Surendranathan N, Kong X, Cyberski N, Garcia JD, Cheng X, Sharma A, Li PK, Larue RC. Development of Murine Leukemia Virus Integrase-Derived Peptides That Bind Brd4 Extra-Terminal Domain as Candidates for Suppression of Acute Myeloid Leukemia. ACS Pharmacol Transl Sci. 2021 Oct 08; 4(5):1628-1638. PMID: 34661079.
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    6. Yoder KE, Rabe AJ, Fishel R, Larue RC. Strategies for Targeting Retroviral Integration for Safer Gene Therapy: Advances and Challenges. Front Mol Biosci. 2021; 8:662331. PMID: 34055882.
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    7. Rebensburg SV, Wei G, Larue RC, Lindenberger J, Francis AC, Annamalai AS, Morrison J, Shkriabai N, Huang SW, KewalRamani V, Poeschla EM, Melikyan GB, Kvaratskhelia M. Sec24C is an HIV-1 host dependency factor crucial for virus replication. Nat Microbiol. 2021 04; 6(4):435-444. PMID: 33649557.
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    8. Larue RC, Xing E, Kenney AD, Zhang Y, Tuazon JA, Li J, Yount JS, Li PK, Sharma A. Rationally Designed ACE2-Derived Peptides Inhibit SARS-CoV-2. Bioconjug Chem. 2021 01 20; 32(1):215-223. PMID: 33356169.
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    9. Durham EL, Balog C, Howie RN, Boyce MA, Arand JR, Warren G, LaRue AC, Cray JJ. Effects of nicotine exposure on murine mandibular development. PLoS One. 2019; 14(6):e0218376. PMID: 31194840.
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    10. McDonald LT, Lopez MF, Helke KL, McCrackin MA, Cray JJ, Becker HC, LaRue AC. Early Blood Profile of C57BL/6 Mice Exposed to Chronic Unpredictable Stress. Front Psychiatry. 2019; 10:230. PMID: 31068843.
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    11. Kelly RR, Mccrackin MA, Russell DL, Leddy LR, Cray JJ, Larue AC. Teaching Surgical Model Development in Research by Using Situated Learning and Instructional Scaffolding. J Am Assoc Lab Anim Sci. 2019 05 01; 58(3):321-328. PMID: 30987688.
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    12. Kelly RR, McDonald LT, Jensen NR, Sidles SJ, LaRue AC. Impacts of Psychological Stress on Osteoporosis: Clinical Implications and Treatment Interactions. Front Psychiatry. 2019; 10:200. PMID: 31024360.
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    13. Brinton DL, Simpson AN, Fominaya CE, LaRue AC. Impact of selective serotonin reuptake inhibitors in the veteran population: 10-year risk outcomes. J Comp Eff Res. 2019 04; 8(6):431-440. PMID: 30855179.
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    14. Grey ZJ, Howie RN, Durham EL, Hall SR, Helke KL, Steed MB, LaRue AC, Muise-Helmericks RC, Cray JJ. Sub-clinical dose of bone morphogenetic protein-2 does not precipitate rampant, sustained inflammatory response in bone wound healing. Wound Repair Regen. 2019 07; 27(4):335-344. PMID: 30805987.
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    15. Sidles SJ, Xiong Y, Young MRI, LaRue AC. High-Fat Diet Alters Immunogenic Properties of Circulating and Adipose Tissue-Associated Myeloid-Derived CD45+DDR2+ Cells. Mediators Inflamm. 2019; 2019:1648614. PMID: 31015794.
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    16. Sun B, Jensen NR, Chung D, Yang M, LaRue AC, Cheung HW, Wang Q. Synergistic effects of SHP2 and PI3K pathway inhibitors in GAB2-overexpressing ovarian cancer. Am J Cancer Res. 2019; 9(1):145-159. PMID: 30755818.
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    17. Durham EL, Howie RN, Hall S, Larson N, Oakes B, Houck R, Grey Z, Steed M, LaRue AC, Muise-Helmericks R, Cray J. Optimizing bone wound healing using BMP2 with absorbable collagen sponge and Talymed nanofiber scaffold. J Transl Med. 2018 11 21; 16(1):321. PMID: 30463618.
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    18. Kelly RR, McDonald LT, Pellegrini VD, Cray JJ, Larue AC. Identification of circulating murine CD34+OCN+ cells. Cytotherapy. 2018 11; 20(11):1371-1380. PMID: 30340982.
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    19. Carrell RK, Stanton RA, Ethier SP, LaRue AC, Soloff AC. ICOSL-augmented adenoviral-based vaccination induces a bipolar Th17/Th1 T cell response against unglycosylated MUC1 antigen. Vaccine. 2018 10 08; 36(42):6262-6269. PMID: 30219366.
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    20. Howie RN, Herberg S, Durham E, Grey Z, Bennfors G, Elsalanty M, LaRue AC, Hill WD, Cray JJ. Selective serotonin re-uptake inhibitor sertraline inhibits bone healing in a calvarial defect model. Int J Oral Sci. 2018 09 03; 10(3):25. PMID: 30174329.
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    21. Wilson KR, Kang IH, Baliga U, Xiong Y, Chatterjee S, Moore E, Parthiban B, Thyagarajan K, Borke JL, Mehrotra S, Kirkwood KL, LaRue AC, Ogawa M, Mehrotra M. Hematopoietic Stem Cells as a Novel Source of Dental Tissue Cells. Sci Rep. 2018 05 23; 8(1):8026. PMID: 29795229.
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    22. Kimbrough D, Wang SH, Wright LH, Mani SK, Kasiganesan H, LaRue AC, Cheng Q, Nadig SN, Atkinson C, Menick DR. HDAC inhibition helps post-MI healing by modulating macrophage polarization. J Mol Cell Cardiol. 2018 06; 119:51-63. PMID: 29680681.
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    23. McDonald LT, Johnson SD, Russell DL, Young MRI, LaRue AC. Role of a novel immune modulating DDR2-expressing population in silica-induced pulmonary fibrosis. PLoS One. 2017; 12(7):e0180724. PMID: 28700752.
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    24. Xiong Y, Russell DL, McDonald LT, Cowart LA, LaRue AC. Hematopoietic Stem Cell-derived Adipocytes Promote Tumor Growth and Cancer Cell Migration. Int J Cancer Res Mol Mech. 2017; 3(1). PMID: 28989976.
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    25. Evans-Knowell A, LaRue AC, Findlay VJ. MicroRNAs and Their Impact on Breast Cancer, the Tumor Microenvironment, and Disparities. Adv Cancer Res. 2017; 133:51-76. PMID: 28052821.
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    26. Lang H, Nishimoto E, Xing Y, Brown LN, Noble KV, Barth JL, LaRue AC, Ando K, Schulte BA. Contributions of Mouse and Human Hematopoietic Cells to Remodeling of the Adult Auditory Nerve After Neuron Loss. Mol Ther. 2016 Nov; 24(11):2000-2011. PMID: 27600399.
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    27. An N, Xiong Y, LaRue AC, Kraft AS, Cen B. Activation of Pim Kinases Is Sufficient to Promote Resistance to MET Small-Molecule Inhibitors. Cancer Res. 2015 Dec 15; 75(24):5318-28. PMID: 26670562.
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    28. Ogawa M, LaRue AC, Mehrotra M. Plasticity of hematopoietic stem cells. Best Pract Res Clin Haematol. 2015 Jun-Sep; 28(2-3):73-80. PMID: 26590762.
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    29. McDonald LT, LaRue AC. Bone Marrow Stem Cell Contribution to Pulmonary Homeostasis and Disease. J Bone Marrow Res. 2015 Sep; 3(3). PMID: 26798846.
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    30. Yang A, Xiao X, Zhao M, LaRue AC, Schulte BA, Wang GY. Differential Reponses of Hematopoietic Stem and Progenitor Cells to mTOR Inhibition. Stem Cells Int. 2015; 2015:561404. PMID: 26221145.
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    31. Van De Voorde L, Janssen L, Larue R, Houben R, Buijsen J, Sosef M, Vanneste B, Schraepen MC, Berbée M, Lambin P. Can metformin improve 'the tomorrow' of patients treated for oesophageal cancer? Eur J Surg Oncol. 2015 Oct; 41(10):1333-9. PMID: 26091848.
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    32. McDonald LT, Russell DL, Kelly RR, Xiong Y, Motamarry A, Patel RK, Jones JA, Watson PM, Turner DP, Watson DK, Soloff AC, Findlay VJ, LaRue AC. Hematopoietic stem cell-derived cancer-associated fibroblasts are novel contributors to the pro-tumorigenic microenvironment. Neoplasia. 2015 May; 17(5):434-48. PMID: 26025666.
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    33. Xiong Y, McDonald LT, Russell DL, Kelly RR, Wilson KR, Mehrotra M, Soloff AC, LaRue AC. Hematopoietic stem cell-derived adipocytes and fibroblasts in the tumor microenvironment. World J Stem Cells. 2015 Mar 26; 7(2):253-65. PMID: 25815113.
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    34. Xiao X, Luo H, Vanek KN, LaRue AC, Schulte BA, Wang GY. Catalase inhibits ionizing radiation-induced apoptosis in hematopoietic stem and progenitor cells. Stem Cells Dev. 2015 Jun 01; 24(11):1342-51. PMID: 25603016.
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    35. Cen B, Xiong Y, Song JH, Mahajan S, DuPont R, McEachern K, DeAngelo DJ, Cortes JE, Minden MD, Ebens A, Mims A, LaRue AC, Kraft AS. The Pim-1 protein kinase is an important regulator of MET receptor tyrosine kinase levels and signaling. Mol Cell Biol. 2014 Jul; 34(13):2517-32. PMID: 24777602.
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    36. Ogawa M, LaRue AC, Mehrotra M. Hematopoietic stem cells are pluripotent and not just "hematopoietic". Blood Cells Mol Dis. 2013 Jun; 51(1):3-8. PMID: 23453528.
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    37. Findlay VJ, LaRue AC, Turner DP, Watson PM, Watson DK. Understanding the role of ETS-mediated gene regulation in complex biological processes. Adv Cancer Res. 2013; 119:1-61. PMID: 23870508.
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    38. McDonald LT, LaRue AC. Hematopoietic stem cell derived carcinoma-associated fibroblasts: a novel origin. Int J Clin Exp Pathol. 2012; 5(9):863-73. PMID: 23119103.
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    39. Mehrotra M, Williams CR, Ogawa M, LaRue AC. Hematopoietic stem cells give rise to osteo-chondrogenic cells. Blood Cells Mol Dis. 2013 Jan; 50(1):41-9. PMID: 22954476.
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    40. Turner DP, Findlay VJ, Moussa O, Semenchenko VI, Watson PM, LaRue AC, Desouki MM, Fraig M, Watson DK. Mechanisms and functional consequences of PDEF protein expression loss during prostate cancer progression. Prostate. 2011 Dec; 71(16):1723-35. PMID: 21446014.
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    41. Findlay VJ, Turner DP, Yordy JS, McCarragher B, Shriver MR, Szalai G, Watson PM, Larue AC, Moussa O, Watson DK. Prostate-Derived ETS Factor Regulates Epithelial-to-Mesenchymal Transition through Both SLUG-Dependent and Independent Mechanisms. Genes Cancer. 2011 Feb; 2(2):120-9. PMID: 21779485.
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    42. Moussa O, LaRue AC, Abangan RS, Williams CR, Zhang XK, Masuya M, Gong YZ, Spyropoulos DD, Ogawa M, Gilkeson G, Watson DK. Thrombocytopenia in mice lacking the carboxy-terminal regulatory domain of the Ets transcription factor Fli1. Mol Cell Biol. 2010 Nov; 30(21):5194-206. PMID: 20823267.
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    43. Jyothi V, Li M, Kilpatrick LA, Smythe N, LaRue AC, Zhou D, Schulte BA, Schmiedt RA, Lang H. Unmyelinated auditory type I spiral ganglion neurons in congenic Ly5.1 mice. J Comp Neurol. 2010 Aug 15; 518(16):3254-71. PMID: 20575058.
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    44. Mehrotra M, Rosol M, Ogawa M, Larue AC. Amelioration of a mouse model of osteogenesis imperfecta with hematopoietic stem cell transplantation: microcomputed tomography studies. Exp Hematol. 2010 Jul; 38(7):593-602. PMID: 20417683.
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    45. Ogawa M, Larue AC, Watson PM, Watson DK. Hematopoietic stem cell origin of connective tissues. Exp Hematol. 2010 Jul; 38(7):540-7. PMID: 20412832.
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    46. Ogawa M, Larue AC, Watson PM, Watson DK. Hematopoietic stem cell origin of mesenchymal cells: opportunity for novel therapeutic approaches. Int J Hematol. 2010 Apr; 91(3):353-9. PMID: 20336396.
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    47. Abangan RS, Williams CR, Mehrotra M, Duncan JD, Larue AC. MCP1 directs trafficking of hematopoietic stem cell-derived fibroblast precursors in solid tumor. Am J Pathol. 2010 Apr; 176(4):1914-26. PMID: 20167869.
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    48. Shirai K, Sera Y, Bulkeley W, Mehrotra M, Moussa O, LaRue AC, Watson DK, Stuart RK, Lazarchick J, Ogawa M. Hematopoietic stem cell origin of human fibroblasts: cell culture studies of female recipients of gender-mismatched stem cell transplantation and patients with chronic myelogenous leukemia. Exp Hematol. 2009 Dec; 37(12):1464-71. PMID: 19786066.
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    49. Sera Y, LaRue AC, Moussa O, Mehrotra M, Duncan JD, Williams CR, Nishimoto E, Schulte BA, Watson PM, Watson DK, Ogawa M. Hematopoietic stem cell origin of adipocytes. Exp Hematol. 2009 Sep; 37(9):1108-20, 1120.e1-4. PMID: 19576951.
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    50. Ogawa M, LaRue AC. Origin of fibroblast colony-forming units. Exp Hematol. 2007 Sep; 35(9):1319-20. PMID: 17637495.
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    51. Ogawa M, LaRue AC, Drake CJ. Hematopoietic origin of fibroblasts/myofibroblasts: Its pathophysiologic implications. Blood. 2006 Nov 01; 108(9):2893-6. PMID: 16840726.
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    52. Visconti RP, Ebihara Y, LaRue AC, Fleming PA, McQuinn TC, Masuya M, Minamiguchi H, Markwald RR, Ogawa M, Drake CJ. An in vivo analysis of hematopoietic stem cell potential: hematopoietic origin of cardiac valve interstitial cells. Circ Res. 2006 Mar 17; 98(5):690-6. PMID: 16456103.
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    53. Ebihara Y, Masuya M, Larue AC, Fleming PA, Visconti RP, Minamiguchi H, Drake CJ, Ogawa M. Hematopoietic origins of fibroblasts: II. In vitro studies of fibroblasts, CFU-F, and fibrocytes. Exp Hematol. 2006 Feb; 34(2):219-29. PMID: 16459190.
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    54. LaRue AC, Masuya M, Ebihara Y, Fleming PA, Visconti RP, Minamiguchi H, Ogawa M, Drake CJ. Hematopoietic origins of fibroblasts: I. In vivo studies of fibroblasts associated with solid tumors. Exp Hematol. 2006 Feb; 34(2):208-18. PMID: 16459189.
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    55. Wang Y, Schulte BA, LaRue AC, Ogawa M, Zhou D. Total body irradiation selectively induces murine hematopoietic stem cell senescence. Blood. 2006 Jan 01; 107(1):358-66. PMID: 16150936.
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    56. LaRue AC, Argraves WS, Zile MH, Drake CJ. Critical role for retinol in the generation/differentiation of angioblasts required for embryonic blood vessel formation. Dev Dyn. 2004 Aug; 230(4):666-74. PMID: 15254901.
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    57. Drake CJ, Fleming PA, Larue AC, Barth JL, Chintalapudi MR, Argraves WS. Differential distribution of cubilin and megalin expression in the mouse embryo. Anat Rec A Discov Mol Cell Evol Biol. 2004 Mar; 277(1):163-70. PMID: 14983511.
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    58. LaRue AC, Lansford R, Drake CJ. Circulating blood island-derived cells contribute to vasculogenesis in the embryo proper. Dev Biol. 2003 Oct 01; 262(1):162-72. PMID: 14512026.
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    59. LaRue AC, Mironov VA, Argraves WS, Czirók A, Fleming PA, Drake CJ. Patterning of embryonic blood vessels. Dev Dyn. 2003 Sep; 228(1):21-9. PMID: 12950076.
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    60. Argraves WS, Larue AC, Fleming PA, Drake CJ. VEGF signaling is required for the assembly but not the maintenance of embryonic blood vessels. Dev Dyn. 2002 Nov; 225(3):298-304. PMID: 12412012.
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    61. Orr RK, Hoehn JL, Col NF. The learning curve for sentinel node biopsy in breast cancer: practical considerations. Arch Surg. 1999 Jul; 134(7):764-7. PMID: 16909203.
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    62. Orr RK, Hoehn JL, Col NF. The learning curve for sentinel node biopsy in breast cancer: practical considerations. Arch Surg. 1999 Jul; 134(7):764-7. PMID: 26185498.
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    63. Orr RK, Hoehn JL, Col NF. The learning curve for sentinel node biopsy in breast cancer: practical considerations. Arch Surg. 1999 Jul; 134(7):764-7. PMID: 23971998.
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    64. Orr RK, Hoehn JL, Col NF. The learning curve for sentinel node biopsy in breast cancer: practical considerations. Arch Surg. 1999 Jul; 134(7):764-7. PMID: 29522370.
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