Using Drosophila as the model system, one of the major goals of my lab is to delineate the molecular mechanisms by which the Piwi-piRNA pathway silences transposons and maintains genome integrity. Our recent work has shown that epigenetic regulation by Piwi prevents trans-generational phenotypic variations. This work has revealed an unprecedented link between Hsp90, a highly selective molecular chaperone and an anti-cancer target, and Piwi. My lab will extend these studies and further unravel the mechanisms by which heat shock machinery regulates Piwi-piRNA pathway. Lastly, we are also interested in understanding if and how the relationship between piRNA pathway and heat shock machinery plays a role in generation of trans-generational epialleles in response to extreme environmental stress. We hope that this study will provide novel insights into stress response pathways and provide us tools to counteract adverse effects of extreme environmental stress and prevent human disease.